SERPINA6/CBG Polyclonal antibody

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  WB analysis of human plasma using 29931-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 29931-1-AP (CBG/Corticosteroid binding globulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 29931-1-AP

  IP result of anti-SERPINA6/CBG (IP:29931-1-AP, 4ug; Detection:29931-1-AP 1:1000) with human placenta tissue lysate 960 ug.

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  IHC staining of human kidney using 29931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29931-1-AP (Corticosteroid binding globulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29931-1-AP (Corticosteroid binding globulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SERPINA6/CBG Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 29931-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

CBG, Serpin A6, SERPINA6, Corticosteroid binding globulin, Corticosteroid-binding globulin

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Tested Applications

• Positive WB detected in: human plasma
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29931-1-AP targets SERPINA6/CBG in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINA6/CBG fusion protein Ag31861
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC058021
• Gene Symbol: SERPINA6
• Gene ID (NCBI): 866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08185
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).