SerpinB2/PAI-2 Polyclonal antibody

• 

  WB analysis using 16035-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16035-1-AP (SERPINB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16035-1-AP

  IP result of anti-SERPINB2 (IP:16035-1-AP, 4ug; Detection:16035-1-AP 1:300) with Jurkat cells lysate 3600ug.

• 

  IHC staining of mouse brain using 16035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SerpinB2/PAI-2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16035-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

PAI 2, PAI-2, SERPINB2, Monocyte Arg-serpin, PAI2

---

Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 3 publications below

Product Information

16035-1-AP targets SerpinB2/PAI-2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8906

  Product name: Recombinant human SERPINB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-415 aa of BC012609

  Sequence: MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKIYSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 2
• Calculated Molecular Weight: 415 aa, 47 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC012609
• Gene Symbol: SERPINB2
• Gene ID (NCBI): 5055
• RRID: AB_2186180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05120
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SerpinB2, a plasminogen activator inhibitor-2 (PAI-2), is a serine protease inhibitor of the serpin superfamily that inactivates tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) (PMID: 7506655). SerpinB2 may have both pro-tumor and anti-tumor effects. High expression of tumor cell-associated SerpinB2 facilitates breast cancer cell survival and metastasis (PMID: 24581498; PMID: 28427146), while SerpinB2 expression by breast cancer cells significantly inhibits metastasis by inhibiting extracellular uPA (PMID: 24644264; PMID: 28346421).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Oncotarget; Exposure to airborne PM2.5 suppresses microRNA expression and deregulates target oncogenes that cause neoplastic transformation in NIH3T3 cells.; Authors - Chunling Liu
	IHC	PLoS One; Plasminogen Activator Inhibitor-2 Plays a Leading Prognostic Role among Protease Families in Non-Small Cell Lung Cancer.; Authors - Chia-Yi Su
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	WB,IHC	Mol Cancer; circPSD3 is a promising inhibitor of uPA system to inhibit vascular invasion and metastasis in hepatocellular carcinoma; Authors - Liangliang Xu
human	WB	Cancer Res; A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer; Authors - Yanchao Yu
	WB	PLoS One; Evaluation and Characterization of Acute respiratory distress syndrome in tree shrews through TMT proteomic method; Authors - Junlong Xiong