Neuroserpin Polyclonal antibody

Neuroserpin Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 12558-1-AP

Synonyms

Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12

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Validation Data Gallery

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  WB analysis of mouse brain using 12558-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12558-1-AP (Neuroserpin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 12558-1-AP

  IP result of anti-Neuroserpin (IP:12558-1-AP, 4ug; Detection:12558-1-AP 1:300) with rat brain tissue lysate 1880 ug.

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  IF Staining of mouse brain using 12558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Neuroserpin antibody (12558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: rat brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12558-1-AP targets Neuroserpin in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neuroserpin fusion protein Ag3275
• Full Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Calculated Molecular Weight: 410 aa, 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC018043
• Gene Symbol: Neuroserpin
• Gene ID (NCBI): 5274
• RRID: AB_10642703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.