SFRS2 Polyclonal antibody

SFRS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21212-1-AP

Synonyms

PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2

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Validation Data Gallery

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  WB analysis using 21212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21212-1-AP (SFRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21212-1-AP targets SFRS2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRS2 fusion protein Ag15612
• Full Name: splicing factor, arginine/serine-rich 2
• Calculated Molecular Weight: 221 aa, 25 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC070086
• Gene Symbol: SFRS2
• Gene ID (NCBI): 6427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS2, also named as PR264 and SC-35, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.