SIRT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

258

Reviews (2)

Cat no : 13161-1-AP

Synonyms

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

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Validation Data Gallery

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  WB analysis using 13161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13161-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 13161-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13161-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13161-1-AP (SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MDA-MB-231 cells, C2C12 cells
• Positive IHC detected in: mouse testis tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 26 publications below
• WB: See 236 publications below
• IHC: See 34 publications below
• IF: See 30 publications below
• IP: See 6 publications below
• ELISA: See 1 publications below
• CoIP: See 5 publications below

Product Information

13161-1-AP targets SIRT1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, bovine, goat, Megalobrama amblycephala, pig, sheep, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT1 fusion protein Ag3808
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Calculated molecular weight: 747 aa, 82 kDa
• Observed molecular weight: 110-130 kDa, 80-85 kDa
• GenBank accession number: BC012499
• Gene symbol: SIRT1
• Gene ID (NCBI): 23411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	WB,IF	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu ; KD Validated
mouse	WB,IHC	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
	IF	J Pineal Res; Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos.; Authors - Ying-Jie Niu
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Uxoa (Verified Customer) (02-19-2020)

Non nuclear signal of SIRT1 in human fibroblast. Cells fixed with 4% PFA 15 min. Ab dilution 1:100 in PBST (0.1% triton)+10% GS. O/N 4ºC incubation. Secondary ab: Goat anti-rabbit alexafluor 555 1:500 in PBST + 10% GS 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human fibroblasts

SIRT1 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:13161-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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