|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||mouse testis tissue, human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13161-1-AP targets SIRT1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, human, Megalobrama amblycephala, mouse, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIRT1 fusion protein Ag3808|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)|
|Calculated molecular weight||747 aa, 82 kDa|
|Observed molecular weight||110-130 kDa, 80-85 kDa|
|GenBank accession number||BC012499|
|Gene ID (NCBI)||23411|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.
|Product Specific Protocols|
|WB protocol for SIRT1 antibody 13161-1-AP||Download protocol|
|IHC protocol for SIRT1 antibody 13161-1-AP||Download protocol|
|IF protocol for SIRT1 antibody 13161-1-AP||Download protocol|
|IP protocol for SIRT1 antibody 13161-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines.
Neuroprotective Effects of Ginsenosides against Cerebral Ischemia.
The protective effect of oleanolic acid on NMDA-induced MLE-12 cells apoptosis and lung injury in mice by activating SIRT1 and reducing NF-κB acetylation.
Bone marrow mesenchymal stem cells could reduce the toxic effects of hexavalent chromium on the liver by decreasing endoplasmic reticulum stress-mediated apoptosis via SIRT1/HIF-1α signaling pathway in rats.
J Nutr Biochem
High fructose diet induces early mortality via autophagy factors accumulation in the rostral ventrolateral medulla as ameliorated by pioglitazone.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Uxoa (Verified Customer) (02-19-2020)
Non nuclear signal of SIRT1 in human fibroblast. Cells fixed with 4% PFA 15 min. Ab dilution 1:100 in PBST (0.1% triton)+10% GS. O/N 4ºC incubation. Secondary ab: Goat anti-rabbit alexafluor 555 1:500 in PBST + 10% GS 1h RT.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution