SIRT1 Polyclonal antibody

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  WB analysis using 13161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13161-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 13161-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13161-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 13161-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13161-1-AP (SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SIRT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13161-1-AP

Publications(402)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

EC:2.3.1.-, EC:2.3.1.286, hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MDA-MB-231 cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 36 publications below
• WB: See 367 publications below
• IHC: See 49 publications below
• IF: See 54 publications below
• IP: See 10 publications below
• ELISA: See 1 publications below
• CoIP: See 9 publications below
• RIP: See 1 publications below

Product Information

13161-1-AP targets SIRT1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, chicken, zebrafish, bovine, sheep, goat, megalobrama amblycephala
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT1 fusion protein Ag3808
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Calculated Molecular Weight: 747 aa, 82 kDa
• Observed Molecular Weight: 110-130 kDa, 80-85 kDa
• GenBank Accession Number: BC012499
• Gene Symbol: SIRT1
• Gene ID (NCBI): 23411
• RRID: AB_10646436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96EB6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT1, also named SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

Publications

Species	Application	Title
	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
	WB	Cell Metab; IgG is an aging factor that drives adipose tissue fibrosis and metabolic decline; Authors - Lexiang Yu
	WB,IF	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu ; KD Validated
	WB	Bioact Mater; Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection; Authors - Yumei Que
	WB,IHC	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
	IF	J Pineal Res; Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos.; Authors - Ying-Jie Niu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Uxoa (Verified Customer) (02-19-2020)

Non nuclear signal of SIRT1 in human fibroblast. Cells fixed with 4% PFA 15 min. Ab dilution 1:100 in PBST (0.1% triton)+10% GS. O/N 4ºC incubation. Secondary ab: Goat anti-rabbit alexafluor 555 1:500 in PBST + 10% GS 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human fibroblasts

SIRT1 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:13161-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain