SIX2 Monoclonal antibody

SIX2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, rat, Canine, mouse, pig







Cat no : 66347-1-Ig


Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

Tested Applications

Positive WB detected inpig skeletal muscle tissue, HEK-293 cells, MDCK cells, rat skeletal muscle tissue, NCCIT cells, L02 cells, mouse skeletal muscle tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66347-1-Ig targets SIX2 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, Canine, mouse, pig samples.

Tested Reactivity human, rat, Canine, mouse, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIX2 fusion protein Ag2124
Full Name SIX homeobox 2
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC024033
Gene Symbol SIX2
Gene ID (NCBI) 10736
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].


Product Specific Protocols
WB protocol for SIX2 antibody 66347-1-IgDownload protocol
IHC protocol for SIX2 antibody 66347-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

Disruption of Gen1 causes ectopic budding and kidney hypoplasia in mice

Authors - Yaxin Li

Dev Biol

Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf.

Authors - Bliss Magella

Dev Dyn

Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling

Authors - Wen Shi

Nucleic Acids Res

The transcriptional coactivator Eya1 exerts transcriptional repressive activity by interacting with REST corepressors and REST-binding sequences to maintain nephron progenitor identity

Authors - Jun Li

J Am Soc Nephrol

Chromatin Remodelers Interact with Eya1 and Six2 to Target Enhancers to Control Nephron Progenitor Cell Maintenance

Authors - Jun Li