SIX2 Monoclonal antibody, PBS Only

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  WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

  Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66347-1-Ig (same clone as 66347-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66347-1-Ig (same clone as 66347-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66347-1-Ig (same clone as 66347-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66347-1-Ig (same clone as 66347-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDCK using 66347-1-Ig (same clone as 66347-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skeletal muscle using 66347-1-Ig (same clone as 66347-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIX2 antibody (66347-1-Ig, Clone: 1F9G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

SIX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66347-1-PBS

Clone No.1F9G11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

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Product Information

66347-1-PBS targets SIX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

• Tested Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2124

  Product name: Recombinant human SIX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-291 aa of BC024033

  Sequence: MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS

• Full Name: SIX homeobox 2
• Calculated Molecular Weight: 291 aa, 32 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC024033
• Gene Symbol: SIX2
• Gene ID (NCBI): 10736
• RRID: AB_2881727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NPC8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].