CoraLite®594-conjugated SIX2 Monoclonal antibody

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  IF Staining of HeLa using CL594-66347

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SIX2 antibody (CL594-66347, Clone: 1F9G11 ) at dilution of 1:1200, CL488-Phalloidin (green).

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  FC experiment of C2C12 using CL594-66347

  1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SIX2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-66347

Clone No.1F9G11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, canine

Applications

IF/ICC, FC (Intra)

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66347 targets SIX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, canine samples.

• Tested Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2124

  Product name: Recombinant human SIX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-291 aa of BC024033

  Sequence: MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS

• Full Name: SIX homeobox 2
• Calculated Molecular Weight: 291 aa, 32 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC024033
• Gene Symbol: SIX2
• Gene ID (NCBI): 10736
• RRID: AB_3084706
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NPC8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].