SLC17A9 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC17A9 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26731-1-AP

Synonyms

C20orf59, SLC17A9, Vesicular nucleotide transporter, VNUT

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Validation Data Gallery

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  WB analysis using 26731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 26731-1-AP

  IP result of anti-SLC17A9 (IP:26731-1-AP, 4ug; Detection:26731-1-AP 1:1000) with mouse brain tissue lysate 4400ug.

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  IHC staining of human skin using 26731-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26731-1-AP (SLC17A9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 26731-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26731-1-AP (SLC17A9 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human pancreas using 26731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 26731-1-AP (SLC17A9 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human pancreas using 26731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 26731-1-AP (SLC17A9 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, C2C12 cells, fetal human brain tissue, mouse brain tissue, Caco-2 cells, THP-1 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

26731-1-AP targets SLC17A9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC17A9 fusion protein Ag24970
• Full Name: solute carrier family 17, member 9
• Calculated Molecular Weight: 436 aa, 47 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC025312
• Gene Symbol: SLC17A9
• Gene ID (NCBI): 63910
• RRID: AB_2880617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	iScience; SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma; Authors - Weiquan Li ; KD Validated
human,mouse	IHC,IF	iScience; A purinergic mechanism underlying metformin regulation of hyperglycemia; Authors - Jared Senfeld
human	WB	Oncol Lett; SLC17A9 expression levels in a pan‑cancer panel and validation of the role of SLC17A9 as a novel prognostic biomarker for osteosarcoma; Authors - Junqing Li ; KD Validated