Mitoferrin 1 Monoclonal antibody

Mitoferrin 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

Unconjugated

CloneNo.

1B1B2

Cat no : 67593-1-Ig

Synonyms

HT015, MFRN, Mitoferrin 1, MSC, MSCP, PRO1278, PRO1584, PRO2217, SLC25A37

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Validation Data Gallery

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  WB analysis of TF-1 using 67593-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67593-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 67593-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67593-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67593-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 67593-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67593-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, HepG2 cells, human spleen tissue, K-562 cells, L02 cells, LNCaP cells, TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67593-1-Ig targets Mitoferrin 1 in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mitoferrin 1 fusion protein Ag26216
• Full Name: solute carrier family 25, member 37
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC132799
• Gene Symbol: SLC25A37
• Gene ID (NCBI): 51312
• RRID: AB_2882800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitoferrin (mfrn, SLC25A37), which is highly expressed in fetal and adult hematopoietic tissues, is a member of the vertebrate mitochondrial solute carrier family (SLC25) and is located on the mitochondrial inner membrane. It functions as an essential and high-affinity iron importer for the biosynthesis of mitochondrial heme and iron-sulfur cluster in vertebrate erythroblasts. Additionally, since it was identified as a major depressive disorder (MDD) risk gene, it is likely to be used as a potential biomarker of MDD diagnose.