SLC35F2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC35F2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 25526-1-AP

Synonyms

HSNOV1, SLC35F2

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Validation Data Gallery

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  WB analysis of A549 using 25526-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25526-1-AP (SLC35F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25526-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25526-1-AP (SLC35F2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: A549 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Product Information

The immunogen of 25526-1-AP is SLC35F2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC35F2 fusion protein Ag20901
• Full Name: solute carrier family 35, member F2
• Calculated Molecular Weight: 374 aa, 41 kDa
• Observed Molecular Weight: 41-50 kDa
• GenBank Accession Number: BC039195
• Gene Symbol: SLC35F2
• Gene ID (NCBI): 54733
• RRID: AB_2880119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC35F2, also named as Solute carrier family 35 member F2, is a 374 amino acid protein, which belongs to the SLC35F solute transporter family. SLC35F2 as a Multi-pass membrane protein is a putative solute transporter. SLC35F2 is highly homologous to the lung squamous cell cancer-related gene, LSCC3, which is highly expressed in lung squamous cell tumour tissues. However, the clinical implication of the SLC35F2 gene in tumour development and progression remains unclear.

Publications

Species	Application	Title
human	WB	Theranostics; USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2; Authors - Arun Pandian Chandrasekaran
human	WB	Mol Cancer Res; Exploiting AR-Regulated Drug Transport to Induce Sensitivity to the Survivin Inhibitor YM155.; Authors - Michael D Nyquist
human	WB,IP	Biochem Biophys Res Commun; βTrCP1 promotes SLC35F2 protein ubiquitination and inhibits cancer progression in HeLa cells; Authors - Jencia Carminha Colaco
human	WB	Onco Targets Ther; Knockdown of SLC35F2 Inhibits the Proliferation and Metastasis of Bladder Cancer Cells.; Authors - Mei Chen ; KD Validated
human	WB	Biochim Biophys Acta Gen Subj; E3 ubiquitin ligase APC/CCdh1 regulates SLC35F2 protein turnover and inhibits cancer progression in HeLa cells; Authors - Jencia Carminha Colaco