SLC37A2 Polyclonal antibody

SLC37A2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20469-1-AP

Synonyms

pp11662, SLC37A2, SPX2, Sugar phosphate exchanger 2

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Validation Data Gallery

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  WB analysis of mouse thymus using 20469-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20469-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 20469-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HeLa cells, RAW 264.7 cells, mouse spleen tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

20469-1-AP targets SLC37A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC37A2 fusion protein Ag14306
• Full Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 2
• Calculated Molecular Weight: 505 aa, 55 kDa
• Observed Molecular Weight: 50-75 kDa
• GenBank Accession Number: BC051314
• Gene Symbol: SLC37A2
• Gene ID (NCBI): 219855
• RRID: AB_10733754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The solute carrier family 37 (SLC37) consists of four sugar-phosphate exchangers, A1, A2, A3, and A4, which are anchored in the endoplasmic reticulum (ER) membrane (PMID:23506893). Solute Carrier Family 37 Member 2 (SLC37A2), also knows as SPX2, was firstly identified in a work, conducted on mice and aimed to detect cAMP inducible genes playing a role in promoting cholesterol efflux from the macrophage cell line RAW264 via apoE and apoA1 (PMID:11004510). Interestingly, of the four SLC37A members, SLC37A2 displays the highest level of transcript abundance in neutrophils and macrophages ,indicating that SLC37A2 may play an essential role in regulating innate immune function (PMID:32428862).

Publications

Species	Application	Title
human	WB,IHC	Cell Biol Toxicol; hucMSC-Ex alleviates inflammatory bowel disease in mice by enhancing M2-type macrophage polarization via the METTL3-Slc37a2-YTHDF1 axis; Authors - Xinwei Xu