SLC40A1/FPN1 Polyclonal antibody

SLC40A1/FPN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

78

Cat no : 26601-1-AP

Synonyms

Ferroportin 1, FPN1, HFE4, IREG1, Iron regulated transporter 1, MST079, MSTP079, MTP1, SLC11A3, SLC40A1

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Validation Data Gallery

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26601-1-AP (SLC40A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26601-1-AP (SLC40A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse small intestine using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse spleen tissue, HepG2 cells, mouse liver tissue, rat spleen, HEK-293T cells, SW480 cells
• Positive IHC detected in: human liver tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Located in the cell membrane and cytoplasm

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 75 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

26601-1-AP targets SLC40A1/FPN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC40A1/FPN1 fusion protein Ag24273
• Full Name: solute carrier family 40 (iron-regulated transporter), member 1
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC037733
• Gene Symbol: SLC40A1/Ferroportin
• Gene ID (NCBI): 30061
• RRID: AB_2880571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC40A1, also named as Ferroportin 1 (FPN1), is an iron transporter which is involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. It has been reported that SLC40A1 mutations may lead to macrophage iron overload, hyperferritinemia, and normal transferrin saturation among ferroportin iron overload patients. This antibody detects 62-70 kDa protein as well as the fragments of 56 kDa and 35 kDa protein similar to papers in SDS-PAGE. (PMID: 21396368,16054062, 18974313)

Publications

Species	Application	Title
human	WB	Sci Adv; Magnetically attracting hydrogel reshapes iron metabolism for tissue repair; Authors - Yichang Xu
human	WB	Redox Biol; NCOA4-mediated ferritinophagy is involved in ionizing radiation-induced ferroptosis of intestinal epithelial cells.; Authors - Hao Zhou
mouse	WB	Sci Total Environ; Effects of chronic low-level lead (Pb) exposure on cognitive function and hippocampal neuronal ferroptosis: An integrative approach using bioinformatics analysis, machine learning, and experimental validation; Authors - Yingsi Cao
human	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
human	WB	Theranostics; Holo-lactoferrin: the link between ferroptosis and radiotherapy in triple-negative breast cancer.; Authors - Zheng Zhang
human	WB	Phytomedicine; Daphnetin induces ferroptosis in ovarian cancer by inhibiting NAD(P)H:Quinone oxidoreductase 1 (NQO1); Authors - Ning Ma