CHT1 Polyclonal antibody

CHT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21848-1-AP

Synonyms

CHT, CHT1, hCHT, SLC5A7

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Validation Data Gallery

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  WB analysis of mouse brain using 21848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21848-1-AP (CHT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 21848-1-AP

  IP result of anti-CHT1 (IP:21848-1-AP, 4ug; Detection:21848-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of rat brain using 21848-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21848-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 21848-1-AP (CHT1 antibody at dilution of 1:50.

• 

  IHC staining of human brain using 21848-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 21848-1-AP (CHT1 antibody at dilution of 1:50.

• 

  IHC staining of rat brain using 21848-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 21848-1-AP is CHT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHT1 fusion protein Ag16136
• Full Name: solute carrier family 5 (choline transporter), member 7
• Calculated Molecular Weight: 580 aa, 63 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC111525
• Gene Symbol: SLC5A7
• Gene ID (NCBI): 60482
• RRID: AB_2878925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Mol Metab; Activation of a non-neuronal cholinergic system in visceral white adipose tissue of obese mice and humans; Authors - Ilenia Severi