SLC6A7 Recombinant antibody

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  IHC staining of mouse brain using 83412-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83412-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 83412-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 83412-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83412-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83412-4-RR against Human SLC6A7 were performed. The affinity constant is below 1 pM.

SLC6A7 Uni-rAb^TM Recombinant Antibody for IHC, ELISA

Cat No. 83412-4-RR

Clone No.240395H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

Sodium-dependent proline transporter, PROT, 240395H5

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Tested Applications

• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83412-4-RR targets SLC6A7 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC6A7 fusion protein Ag34413
• Full Name: solute carrier family 6 (neurotransmitter transporter, L-proline), member 7
• Calculated Molecular Weight: 636 aa, 71 kDa
• GenBank Accession Number: BC093785
• Gene Symbol: SLC6A7
• Gene ID (NCBI): 6534
• RRID: AB_3671059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q99884
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC6A7 also known as PROT, belongs to the solute Carrier 6 gene family. SLC6A7 is a high-affinity mammalian brain L-proline transporter, which is different from other sodium-dependent plasma membrane carriers, and has unique pharmacological specificity, kinetic properties, and ionic requirements. SLC6A7 is a brain specific sodium (and chloride)-dependent proline transporter, that terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals(PMID: 7651355).