SMAD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMAD4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 51144-1-AP

Synonyms

DPC4, hSMAD4, JIP, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

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Validation Data Gallery

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  WB analysis of HeLa using 51144-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 cells using 51144-1-AP

  WB results of SMAD4 antibody (51144-1-AP, 1:1000) with si-Control and si-SMAD4 transfected HepG2 cells.

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  WB analysis of PC-3 using 51144-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human placenta tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

51144-1-AP targets SMAD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 4
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC002379
• Gene Symbol: SMAD4
• Gene ID (NCBI): 4089
• RRID: AB_10918273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide (25-43aa) mapping within human SMAD4. It is specific to SMAD4.

Publications

Species	Application	Title
rat	WB	Exp Ther Med; Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation.; Authors - Ruichen Wang
rat	WB	Open Life Sci; NR4A1 inhibits the epithelial-mesenchymal transition of hepatic stellate cells: Involvement of TGF-β-Smad2/3/4-ZEB signaling.; Authors - Qian Huang
rat	WB	Exp Ther Med; MicroRNA-122 inhibits epithelial-mesenchymal transition of hepatic stellate cells induced by the TGF-β1/Smad signaling pathway.; Authors - Bianqiao Cheng