SMIM1 Polyclonal antibody

SMIM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27849-1-AP

Synonyms

hypothetical LOC388588, LOC388588, Small integral membrane protein 1, SMIM1

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Validation Data Gallery

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  WB analysis of HEK-293 using 27849-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 27849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27849-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

27849-1-AP targets SMIM1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMIM1 fusion protein Ag27028
• Full Name: hypothetical LOC388588
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC146957
• Gene Symbol: SMIM1
• Gene ID (NCBI): 388588
• RRID: AB_2880993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small integral membrane protein 1(SMIM1) is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus. SMIM1 is highly expressed in hematopoietic cells. It is associated with a variety of immune responses and plays an important role in RBC differentiation. SMIM1 can be used as a potential biomarker for the diagnosis of IDD (PMID: 30906890; 26452714).

Publications

Species	Application	Title
human	WB	Transfus Med Hemother; CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene; Authors - Jiaxuan Yang ; KO Validated