SMO Monoclonal antibody

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  WB analysis using 66851-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66851-1-Ig (SMO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66851-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66851-1-Ig (SMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66851-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66851-1-Ig (SMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 66851-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66851-1-Ig (SMO antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

SMO Monoclonal Antibody for WB, IF, IHC, ELISA

Cat No. 66851-1-Ig

Clone No.2B10A11

Publications(25)

Reviews (2)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Canine, Mouse, Rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 24 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66851-1-Ig targets SMO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Canine, Mouse, Rat samples.

• Tested Reactivity: Human, Canine, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25861

  Product name: Recombinant human SMO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 28-233 aa of BC009989

  Sequence: RGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSY

• Full Name: smoothened homolog (Drosophila)
• Calculated Molecular Weight: 787 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC009989
• Gene Symbol: SMO
• Gene ID (NCBI): 6608
• RRID: AB_2882191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99835
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Int J Biol Sci; Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma; Authors - Wenjiao Jin
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Front Cell Dev Biol; Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.; Authors - Jiawei Li
rat	WB	Oxid Med Cell Longev; Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats; Authors - Xiaoqiong Li
human	WB	Cancer Sci; Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition.; Authors - Kaibo Guo
human	WB	FASEB J; Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway; Authors - Junjie Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aidan (Verified Customer) (08-31-2021)

Smo Monoclonal antibody responded to S. purpuratus Smo protein in both IF and WB experiments. For IF, methanol fixed embryos treated with 1:100 dilution of the antibody in PBS overnight, followed by incubation in a 1:300 dilution of secondary antibody Alexa Goat anti-mouse 488 diluted 1:300 in PBS. Localization appears peri-nuclear at the 16 cell stage.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: IF: 1:100 WB: 1:2000
• Cell Tissue Type: Strongylocentrotus purpuratus (Purple Sea Urchin) Embryo

SMO Antibody Western Blot,Immunofluorescence validation (IF: 1:100 WB: 1:2000 dilution) in Strongylocentrotus purpuratus (Purple Sea Urchin) Embryo (Cat no:66851-1-Ig)

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody primarily labeled a band around 110 kD; by IF, it cannot label the ciliary localisation of Smo when the cells were stimulated for Hh pathway.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF