CoraLite® Plus 488-conjugated SMO Monoclonal antibody

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  IF Staining of MDCK using CL488-66851

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL488-66851 (SMO antibody) at dilution of 1:100.

SMO Monoclonal Antibody for IF/ICC

Cat No. CL488-66851

Clone No.2B10A11

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Canine

Applications

IF/ICC

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66851 targets SMO in IF/ICC applications and shows reactivity with Human, Canine samples.

• Tested Reactivity: Human, Canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25861

  Product name: Recombinant human SMO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 28-233 aa of BC009989

  Sequence: RGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSY

• Full Name: smoothened homolog (Drosophila)
• Calculated Molecular Weight: 787 aa, 86 kDa
• GenBank Accession Number: BC009989
• Gene Symbol: SMO
• Gene ID (NCBI): 6608
• RRID: AB_2883387
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99835
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.