SNRPE Polyclonal antibody

SNRPE Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20407-1-AP

Synonyms

snRNP-E, snRNP E, Sm-E, SME, Sm protein E

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Validation Data Gallery

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  WB analysis using 20407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20407-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20407-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse placenta tissue, human placenta tissue, human brain tissue
• Positive IHC detected in: mouse brain tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20407-1-AP targets SNRPE in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPE fusion protein Ag14227
• Full Name: small nuclear ribonucleoprotein polypeptide E
• Calculated Molecular Weight: 92 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC002639
• Gene Symbol: SNRPE
• Gene ID (NCBI): 6635
• RRID: AB_10699882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The snRNP E (SNRPE)protein is one of several proteins associated with the U family of small nuclear RNAs that are involved in RNA processing. It functions in the U7 snRNP complex that is involved in histone 3'-end processing and also associated with snRNP U1, U2, U4/U6 and U5. This antibody raises against the full length of human SNRPE, and can recognize two bands of SNRPE, include a 9 kDa band(isoform) and a 11kDa band(SNRPE) (PMID:23246290)

Publications

Species	Application	Title
human	IF	Am J Hum Genet; Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex.; Authors - Sandra M Pasternack
human	WB,IHC	J Pathol; A novel protein-based prognostic signature improves risk stratification to guide clinical management in early-stage lung adenocarcinoma patients.; Authors - Elena Martínez-Terroba