SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F10G1

Cat no : 67480-1-Ig

Synonyms

ALS, ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse brain tissue, pig brain tissue, rat brain tissue, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67480-1-Ig targets SOD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOD1 fusion protein Ag28553
Full Name superoxide dismutase 1, soluble
Calculated molecular weight 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC001034
Gene symbol SOD1
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 67480-1-IgDownload protocol
IHC protocol for SOD1 antibody 67480-1-IgDownload protocol
IF protocol for SOD1 antibody 67480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury.

Authors - Haoyang Ma