SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

2F10G1

Cat no : 67480-1-Ig

Synonyms

ALS, ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

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Validation Data Gallery

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  WB analysis using 67480-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67480-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67480-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67480-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67480-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67480-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67480-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67480-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67480-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293T using 67480-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse brain tissue, pig brain tissue, rat brain tissue, K-562 cells, HSC-T6 cells
• Positive IHC detected in: human liver cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

67480-1-Ig targets SOD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOD1 fusion protein Ag28553
• Full Name: superoxide dismutase 1, soluble
• Calculated molecular weight: 16 kDa
• Observed molecular weight: 16-20 kDa
• GenBank accession number: BC001034
• Gene symbol: SOD1
• Gene ID (NCBI): 6647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Publications

Species	Application	Title
rat	WB	Stem Cell Res Ther; Insufficient S-adenosylhomocysteine hydrolase compromises the beneficial effect of diabetic BMSCs on diabetic cardiomyopathy; Authors - Ying Wang
mouse	IF	Oxid Med Cell Longev; Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2; Authors - Jie Jin
rat	WB	Oxid Med Cell Longev; Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.; Authors - Honglin Liu
human	WB	Thorac Cancer; Icaritin alleviates docetaxel-induced skin injury by suppressing reactive oxygen species via estrogen receptors; Authors - Jie Zou
mouse	WB	Front Pharmacol; Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury.; Authors - Haoyang Ma
mouse	WB	Phytomedicine; β-Lapachone, an NQO1 activator, alleviates diabetic cardiomyopathy by regulating antioxidant ability and mitochondrial function.; Authors - Pei-Yu Wu