SOD1 Monoclonal antibody
SOD1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2F10G1
Cat no : 67480-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse brain tissue, pig brain tissue, rat brain tissue, K-562 cells, HSC-T6 cells |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
67480-1-Ig targets SOD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SOD1 fusion protein Ag28553 |
| Full Name | superoxide dismutase 1, soluble |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16-20 kDa |
| GenBank accession number | BC001034 |
| Gene symbol | SOD1 |
| Gene ID (NCBI) | 6647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOD1 antibody 67480-1-Ig | Download protocol |
| IHC protocol for SOD1 antibody 67480-1-Ig | Download protocol |
| IF protocol for SOD1 antibody 67480-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2 | ||
Oxid Med Cell Longev Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. | ||
Thorac Cancer Icaritin alleviates docetaxel-induced skin injury by suppressing reactive oxygen species via estrogen receptors | ||
Front Pharmacol Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury. |
