CoraLite®594-conjugated SOD1 Monoclonal antibody

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  IF Staining of HEK-293 using CL594-67480

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 SOD1 antibody (CL594-67480, Clone: 2F10G1 ) at dilution of 1:200.

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  FC experiment of HEK-293T using CL594-67480

  1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOD1 (CL594-67480, Clone:2F10G1) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

SOD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-67480

Clone No.2F10G1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

2F10G1, ALS1, EC:1.15.1.1, homodimer, hSod1

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67480 targets SOD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28553

  Product name: Recombinant human SOD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-154 aa of BC001034

  Sequence: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

• Full Name: superoxide dismutase 1, soluble
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC001034
• Gene Symbol: SOD1
• Gene ID (NCBI): 6647
• RRID: AB_3084771
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00441
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.