SOD1 Monoclonal antibody, PBS Only

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  WB analysis using 67480-1-Ig (same clone as 67480-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67480-1-Ig (same clone as 67480-1-PBS)

  HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 67480-1-Ig incubated at 1:10000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 67480-1-Ig (same clone as 67480-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67480-1-Ig (same clone as 67480-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67480-1-Ig (same clone as 67480-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67480-1-Ig (same clone as 67480-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67480-1-Ig (same clone as 67480-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67480-1-Ig (same clone as 67480-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67480-1-Ig (same clone as 67480-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67480-1-Ig (same clone as 67480-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293T using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 67480-1-Ig (same clone as 67480-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 67480-1-Ig (same clone as 67480-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human SOD1 (67480-1-Ig, Clone:2F10G1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SOD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 67480-1-PBS

Clone No.2F10G1

Host / Isotype

Mouse / IgG2a

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

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Product Information

67480-1-PBS targets SOD1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

• Tested Reactivity: rabbit, pig, rat, mouse, human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28553

  Product name: Recombinant human SOD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-154 aa of BC001034

  Sequence: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

• Full Name: superoxide dismutase 1, soluble
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC001034
• Gene Symbol: SOD1
• Gene ID (NCBI): 6647
• RRID: AB_2882707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00441
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643). SOD1 can be detected 24, 32, 40 and 48 kDa by ubiquitination (PMID: 16943203).