SPRED2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPRED2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24091-1-AP

Synonyms

Sprouty-related, EVH1 domain-containing protein 2, Spred-2

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Validation Data Gallery

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  WB analysis of MCF-7 using 24091-1-AP

  WB result of SPRED2 antibody (24091-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPRED2 transfected MCF-7 cells.

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  WB analysis using 24091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24091-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24091-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 24091-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24091-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24091-1-AP (SPRED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-12 cells, MCF-7 cells, mouse testis tissue, mouse brain tissue, rat brain tissue, T-47D cells, rat testis tissue
• Positive IHC detected in: rat testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

24091-1-AP targets SPRED2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRED2 fusion protein Ag21359
• Full Name: sprouty-related, EVH1 domain containing 2
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC136334
• Gene Symbol: SPRED2
• Gene ID (NCBI): 200734
• RRID: AB_2879429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPRED2 is a member of the SPRED family of proteins that modulate GFR signaling by inhibiting the Ras-MAPK cascade (PMID: 17094949). SPRED family members are characterized by an N-terminal Enabled/VASP homology 1 domain (EVH1), a central c-Kit binding domain (KBD) and a C-terminal Sprouty-related domain (SPR) (PMID: 17691106). Three mammalian SPREDs (SPRED1-3) have been described. In adult tissues, SPRED2 is expressed ubiquitously, but most highly expressed in glandular epithelia (PMID: 15580519; 17691106). Reduced expression level of SPRED2 has been reported in human hepatocellular carcinoma (HCC), suggesting SPRED2 may play a role in tumorgenesis (PMID: 16652141). Gene disruption of SPRED2 causes dwarfism (PMID: 15946934).

Publications

Species	Application	Title
human	IHC	PLoS One; Expression of Spred2 in the urothelial tumorigenesis of the urinary bladder; Authors - Shinsuke Oda
mouse	WB	Biol Pharm Bull; Inhibition of Spred/Sprouty Expression in the Skin of a Contact Dermatitis-Like Model.; Authors - Hiroyasu Sakai
mouse	IF	Histol Histopathol; microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2; Authors - Liang Zhu
human	WB	Int J Mol Sci; SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells; Authors - Tong Gao
human,mouse	WB,IHC	Int J Mol Sci; SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular Carcinoma Cells and Normal Hepatocytes; Authors - Tianyi Wang ; KO Validated