SQLE Polyclonal antibody

• Featured Product
• KD/KO Validated

SQLE Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

87

Cat no : 12544-1-AP

Synonyms

Squalene monooxygenase, squalene epoxidase, ERG1, EC:1.14.14.17

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Validation Data Gallery

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  WB analysis using 12544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12544-1-AP (SQLE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12544-1-AP

  IP result of anti-SQLE (IP:12544-1-AP, 4ug; Detection:12544-1-AP 1:500) with HepG2 cells lysate 2240 ug.

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  IHC staining of human prostate cancer using 12544-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12544-1-AP (SQLE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12544-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12544-1-AP (SQLE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12544-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQLE antibody (12544-1-AP) at dilution of 1:40 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PC-3 using 12544-1-AP

  Immunofluorescent analysis of PC-3 cells using 12544-1-AP (SQLE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 16 publications below
• WB: See 78 publications below
• IHC: See 17 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below

Product Information

12544-1-AP targets SQLE in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SQLE fusion protein Ag3266
• Full Name: squalene epoxidase
• Calculated Molecular Weight: 574 aa, 64 kDa
• Observed Molecular Weight: 50-64 kDa
• GenBank Accession Number: BC017033
• Gene Symbol: SQLE
• Gene ID (NCBI): 6713
• RRID: AB_2195888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
hamster	WB	Cell Metab; Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase.; Authors - Gill Saloni S
mouse	WB	Cell Metab; PMID: 21109190; Authors - Ryo Suzuki
mouse	WB	PLoS Biol; Reduction of the cholesterol sensor SCAP in the brains of mice causes impaired synaptic transmission and altered cognitive function.; Authors - Suzuki Ryo R
human	WB,IHC	Nat Commun; MiR-205-driven downregulation of cholesterol biosynthesis through SQLE-inhibition identifies therapeutic vulnerability in aggressive prostate cancer.; Authors - C Kalogirou
human	WB	Redox Biol; Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation; Authors - Youzi Kong

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