ST14 Polyclonal antibody, PBS Only

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  WB analysis using 27176-1-AP (same clone as 27176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 27176-1-AP (same clone as 27176-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 27176-1-AP (same clone as 27176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 27176-1-AP (same clone as 27176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

ST14 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27176-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:3.4.21.109, Matriptase, Membrane-type serine protease 1, MT-SP1, Prostamin

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Product Information

27176-1-PBS targets ST14 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26007

  Product name: Recombinant human ST14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 301-539 aa of BC030532

  Sequence: YNLTFHSSQNVLLITLITNTERRHPGFEATFFQLPRMSSCGGRLRKAQGTFNSPYYPGHYPPNIDCTWNIEVPNNQHVKVRFKFFYLLEPGVPAGTCPKDYVEINGEKYCGERSQFVVTSNSNKITVRFHSDQSYTDTGFLAEYLSYDSSDPCPGQFTCRTGRCIRKELRCDGWADCTDHSDELNCSCDAGHQFTCKNKFCKPLFWVCDSVNDCGDNSDEQGCSCPAQTFRCSNGKCLS

• Full Name: suppression of tumorigenicity 14 (colon carcinoma)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC030532
• Gene Symbol: ST14
• Gene ID (NCBI): 6768
• RRID: AB_2880788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Y6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.