ST14 Polyclonal antibody

ST14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27176-1-AP

Synonyms

HAI, Matriptase, MT SP1, MTSP 1, MTSP1, Prostamin, PRSS14, Serine protease 14, Serine protease TADG 15, SNC19, ST14, TADG 15, TADG15

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Validation Data Gallery

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  WB analysis of MCF-7 using 27176-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 27176-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 27176-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 27176-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 27176-1-AP is ST14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST14 fusion protein Ag26007
• Full Name: suppression of tumorigenicity 14 (colon carcinoma)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC030532
• Gene Symbol: ST14
• Gene ID (NCBI): 6768
• RRID: AB_2880788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	BMC Cancer; ST14 interacts with TMEFF1 and is a predictor of poor prognosis in ovarian cancer; Authors - Xin Nie