ST14 Polyclonal antibody

ST14 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27176-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

EC:3.4.21.109, Matriptase, Membrane-type serine protease 1, MT-SP1, Prostamin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 27176-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 27176-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 27176-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 27176-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 27176-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 27176-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 27176-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	LNCaP cells, MCF-7 cells, mouse colon tissue, rat colon tissue
Positive IHC detected in	human colon tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27176-1-AP targets ST14 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26007 Product name: Recombinant human ST14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 301-539 aa of BC030532 Sequence: YNLTFHSSQNVLLITLITNTERRHPGFEATFFQLPRMSSCGGRLRKAQGTFNSPYYPGHYPPNIDCTWNIEVPNNQHVKVRFKFFYLLEPGVPAGTCPKDYVEINGEKYCGERSQFVVTSNSNKITVRFHSDQSYTDTGFLAEYLSYDSSDPCPGQFTCRTGRCIRKELRCDGWADCTDHSDELNCSCDAGHQFTCKNKFCKPLFWVCDSVNDCGDNSDEQGCSCPAQTFRCSNGKCLS Predict reactive species
Full Name	suppression of tumorigenicity 14 (colon carcinoma)
Calculated Molecular Weight	94.7 kDa
Observed Molecular Weight	100 kDa
GenBank Accession Number	BC030532
Gene Symbol	ST14
Gene ID (NCBI)	6768
RRID	AB_2880788
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5Y6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.

Protocols

Product Specific Protocols
IHC protocol for ST14 antibody 27176-1-AP	Download protocol
WB protocol for ST14 antibody 27176-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ST14 Polyclonal antibody (Cat# 27176-1-AP) has 1 total citation. It was cited in BMC Cancer (IF 4.1), a 2024 study by Xin Nie et al. investigating ST14's interaction with TMEFF1 as a predictor of poor prognosis in ovarian cancer. The research field is oncology, specifically ovarian cancer prognosis and biomarker discovery. The antibody was applied in WB, IHC, IF, and CoIP in human samples.