STAR Polyclonal antibody

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  WB analysis using 12225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12225-1-AP

  Pig adrenal gland lysates were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12225-1-AP

  WB result of STAR antibody (12225-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAR transfected K-562 cells.

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  WB analysis of human adrenal gland using 12225-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using STAR antibody (12225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 12225-1-AP (STAR antibody) at dilution of 1:400 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green).

• KD/KO Validated

STAR Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 12225-1-AP

Publications(88)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (4)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

STARD1, START domain-containing protein 1

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Tested Applications

• Positive WB detected in: mouse testis tissue, human adrenal gland tissue, K-562 cells, pig adrenal gland tissue, rat ovary tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue, human liver cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF-Fro detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 72 publications below
• IHC: See 13 publications below
• IF: See 18 publications below

Product Information

12225-1-AP targets STAR in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2862

  Product name: Recombinant human STAR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-285 aa of BC010550

  Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• RRID: AB_2115832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49675
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Transl Res; Induction of autophagy by Beclin-1 in granulosa cells contributes to follicular progesterone elevation in ovarian endometriosis.; Authors - Ying Ding
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
mouse	WB	Ecotoxicol Environ Saf; FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice; Authors - Lipeng Li
mouse	WB	Environ Pollut; Prenatal exposure to propylparaben at human-relevant doses accelerates ovarian aging in adult mice.; Authors - Milu Li
pig	WB	Biol Res; Unraveling the impact of hyperleptinemia on female reproduction: insights from transgenic pig model; Authors - Muhammad Ameen Jamal