CoraLite® Plus 488-conjugated STAR Polyclonal antibody

STAR Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12225

Synonyms

STAR, STARD1

---

Validation Data Gallery

• 

  IF Staining of mouse testis using CL488-12225

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 STAR antibody (CL488-12225) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-12225

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 STAR antibody (CL488-12225) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12225 targets STAR in IF applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAR fusion protein Ag2862
• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• RRID: AB_2919049
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.