STAR Monoclonal antibody

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  WB analysis of pig adrenal gland using 67130-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67130-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 67130-1-Ig

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67130-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat adrenal gland using 67130-1-Ig

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 67130-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67130-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67130-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 67130-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67130-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67130-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 67130-1-Ig (STAR antibody) at dilution of 1:400, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green).

STAR Monoclonal Antibody for WB, IF-P, IF-Fro, ELISA

Cat No. 67130-1-Ig

Clone No.1G6E6

Publications(12)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

1G6E6, STARD1, START domain-containing protein 1

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Tested Applications

• Positive WB detected in: pig adrenal gland tissue, human adrenal gland tissue, human testis tissue, K-562 cells, rat adrenal gland tissue, rat testis tissue, THP-1 cells, Mo7e cells
• Positive IF-P detected in: mouse testis tissue
• Positive IF-Fro detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

67130-1-Ig targets STAR in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28698

  Product name: Recombinant human STAR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-285 aa of BC010550

  Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• RRID: AB_2882429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49675
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	IF	Theriogenology; Impacts of elevated temperature on morphology, oxidative stress levels, and testosterone synthesis in ex vivo cultured porcine testicular tissue; Authors - Kai Wang
mouse	WB	Mol Cell Endocrinol; SP600125 decreases cAMP/PKA-dependent steroid production through ATF4/DDIT3 activation in MA-10 Leydig cells; Authors - Audrey Basque
mouse	IF	Drug Des Devel Ther; Semaglutide Alleviates Ovary Inflammation via the AMPK/SIRT1/NF‑κB Signaling Pathway in Polycystic Ovary Syndrome Mice; Authors - Mei Liu
		Cell Rep; High matrix stiffness triggers testosterone decline in aging males by disrupting stem Leydig cell pool homeostasis; Authors - Jiayu Huang
mouse	WB	Andrology; Peroxiredoxin 4 Involved in Spermatogenesis by Affecting Oxidative Stress and Ferroptosis.; Authors - Shuning Yuan
mouse	IF	Mol Cell Endocrinol; HE4 overexpression in mice leads to leydig cell hyperplasia and spermatogensis impairment: Pathological implications for oligospermia; Authors - Pengyun Ling