STAR Monoclonal antibody, PBS Only

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  WB analysis of pig adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67130-1-Ig (same clone as 67130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 67130-1-Ig (same clone as 67130-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67130-1-Ig (same clone as 67130-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67130-1-Ig (same clone as 67130-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 67130-1-Ig (same clone as 67130-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 67130-1-Ig (STAR antibody) at dilution of 1:400, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

STAR Monoclonal Antibody for WB, IF-P, IF-Fro, Indirect ELISA

Cat No. 67130-1-PBS

Clone No.1G6E6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, IF-Fro, Indirect ELISA

Synonyms

1G6E6, STARD1, START domain-containing protein 1

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Product Information

67130-1-PBS targets STAR in WB, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28698

  Product name: Recombinant human STAR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-285 aa of BC010550

  Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• RRID: AB_2882429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49675
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue