SUPT5H Polyclonal antibody

• Featured Product
• KD/KO Validated

SUPT5H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16511-1-AP

Synonyms

SPT5, hSPT5, DSIF p160, DSIF large subunit, DRB sensitivity-inducing factor large subunit

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Validation Data Gallery

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  WB analysis of HeLa using 16511-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16511-1-AP (SUPT5H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16511-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16511-1-AP (SUPT5H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16511-1-AP

  IP result of anti-SUPT5H (IP:16511-1-AP, 4ug; Detection:16511-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of mouse testis using 16511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16511-1-AP (SUPT5H antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16511-1-AP (SUPT5H antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16511-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUPT5H antibody (16511-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16511-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUPT5H antibody (16511-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

16511-1-AP targets SUPT5H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUPT5H fusion protein Ag9728
• Full Name: suppressor of Ty 5 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1087 aa, 121 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC024203
• Gene Symbol: SUPT5H
• Gene ID (NCBI): 6829
• RRID: AB_2878268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPT5H, also named as DSIF p160, is a 1087 amino acid protein, which belongs to the SPT5 family. SUPT5H localizes in the nucleus and is ubiquitously expressed. SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H is modified through phosphorylation and methylated, so the molecular weight of SUPT5H might be 140-150 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu ; KD Validated
human	WB	Mol Cell; INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II; Authors - Shibin Hu
		Mol Cell; Three-step mechanism of promoter escape by RNA polymerase II; Authors - Yumeng Zhan
		Mol Cell; CDK7 kinase activity promotes RNA polymerase II promoter escape by facilitating initiation factor release; Authors - Taras Velychko