CoraLite®594-conjugated SYK Monoclonal antibody

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  FC experiment of K-562 using CL594-66721

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SYK (CL594-66721, Clone:4C4A12) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

SYK Monoclonal Antibody for FC (Intra)

Cat No. CL594-66721

Clone No.4C4A12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

4C4A12, EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase

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Tested Applications

• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66721 targets SYK in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17654

  Product name: Recombinant human SYK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 239-427 aa of BC001645

  Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL

• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 635 aa, 72 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• ENSEMBL Gene ID: ENSG00000165025
• RRID: AB_3084723
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43405
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.