Serotonin transporter Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84844-3-RR (same clone as 84844-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84844-3-RR (Serotonin transporter antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84844-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 84844-3-RR (same clone as 84844-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84844-3-RR (Serotonin transporter antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 84844-3-PBS in a different storage buffer formulation.

Serotonin transporter Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84844-3-PBS

Clone No.242252B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

SLC6A4, 242252B9, 5 HTT, 5 HTTLPR, 5HTT

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Product Information

84844-3-PBS targets Serotonin transporter in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_001045
• Gene Symbol: Serotonin transporter
• Gene ID (NCBI): 6532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31645
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, partly by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).