Serotonin transporter Recombinant monoclonal antibody

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  WB analysis of mouse brain using 84844-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84844-3-RR (Serotonin transporter antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 84844-3-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84844-3-RR (Serotonin transporter antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 84844-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84844-3-RR (Serotonin transporter antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse lung using 84844-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84844-3-RR (Serotonin transporter antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

Serotonin transporter Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84844-3-RR

Clone No.242252B9

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SLC6A4, 242252B9, 5 HTT, 5 HTTLPR, 5HTT

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84844-3-RR targets Serotonin transporter in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_001045
• Gene Symbol: Serotonin transporter
• Gene ID (NCBI): 6532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, partly by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sai Sindhura (Verified Customer) (03-23-2026)

Serotonin antibody works very nice

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000

FH

Iram (Verified Customer) (03-23-2026)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for IB and for IHC and IF 1:100
• Cell Tissue Type: Colon cancer