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CoraLite® Plus 488-conjugated Stomatin Monoclonal antibody

Stomatin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4D6

Cat no : CL488-67894

Synonyms

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated Stomatin Monoclonal (CL488-67894)

    FC experiment of A549 using CL488-67894

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Stomatin (CL488-67894, Clone:1A4D6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67894 targets Stomatin in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Stomatin fusion protein Ag2676
Full Name stomatin
Calculated Molecular Weight 288 aa, 32 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC010703
Gene Symbol STOM
Gene ID (NCBI) 2040
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Stomatin antibody CL488-67894Download protocol
Standard Protocols
Click here to view our Standard Protocols