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CoraLite® Plus 488-conjugated Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17785

Synonyms

synaptophysin, SYP

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 488-conjugated Synaptophysin Polycl (CL488-17785)

    IF Staining of mouse pancreas using CL488-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 488-conjugated Synaptophysin Polycl (CL488-17785)

    IF Staining of mouse pancreas using CL488-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Polycl (CL488-17785)

    IF Staining of mouse brain using CL488-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:1000.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Polycl (CL488-17785)

    IF Staining of mouse brain using CL488-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:1000.

Tested Applications

Positive IF detected inmouse pancreas tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17785 targets Synaptophysin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781
Full Name synaptophysin
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC064550
Gene Symbol SYP
Gene ID (NCBI) 6855
RRIDAB_2919155
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Synaptophysin antibody CL488-17785Download protocol
Standard Protocols
Click here to view our Standard Protocols