Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 17785-1-AP

Synonyms

SYN, synaptophysin, SYP



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human pituitary adenoma tissue, ganglioma tissue, neuroblastoma tissue, human brain tissue, human pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, PC-12 cells, human lung cancer tissue, mouse brain tissue, human gliomas tissue
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17785-1-AP targets Synaptophysin in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC064550
Gene symbol SYP
Gene ID (NCBI) 6855
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
WB protocol for Synaptophysin antibody 17785-1-APDownload protocol
IHC protocol for Synaptophysin antibody 17785-1-APDownload protocol
IF protocol for Synaptophysin antibody 17785-1-APDownload protocol
IP protocol for Synaptophysin antibody 17785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol

Cadmium induces actin cytoskeleton alterations and dysfunction in Neuro-2a cells.

Authors - Yaming Ge
mouseIF

Cell

A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets.

Authors - Nadav Sharon
ratWB

Front Psychiatry

Brain-Derived Neurotrophic Factor Precursor in the Hippocampus Regulates Both Depressive and Anxiety-Like Behaviors in Rats.

Authors - Feng Zhong
hamsterWB

Int J Mol Sci

PMID: 30845718

Authors - Syed Zahid Ali Shah
mouseWB

Mol Brain

Absence of BBSome function leads to astrocyte reactivity in the brain.

Authors - Minati Singh
mouseWB

Cells

MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model.

Authors - Jin Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human primary cortical neurons
Synaptophysin Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical neurons (Cat no:17785-1-AP)