Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17785-1-AP

Synonyms

SYN, synaptophysin, SYP



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, ganglioma tissue, human brain tissue, human pituitary adenoma tissue, human pancreas tissue, neuroblastoma tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, PC-12 cells, human lung cancer tissue, mouse brain tissue, human gliomas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17785-1-AP targets Synaptophysin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC064550
Gene symbol SYP
Gene ID (NCBI) 6855
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
WB protocol for Synaptophysin antibody 17785-1-APDownload protocol
IHC protocol for Synaptophysin antibody 17785-1-APDownload protocol
IF protocol for Synaptophysin antibody 17785-1-APDownload protocol
IP protocol for Synaptophysin antibody 17785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse, ratWB,IF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
mouseIF

Mol Cell

Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.

Authors - Zhengxin Ying
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseIF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard
humanWB,IF

Sci Adv

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Authors - Qingzhe Wu
mouseWB

Sci Adv

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission

Authors - Pengcheng Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human primary cortical neurons
Synaptophysin Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical neurons (Cat no:17785-1-AP)