|Positive WB detected in||mouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue, human pituitary adenoma tissue, ganglioma tissue, neuroblastoma tissue, human brain tissue, human pancreas tissue, mouse pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse pancreas tissue, PC-12 cells, human lung cancer tissue, mouse brain tissue, human gliomas tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
17785-1-AP targets Synaptophysin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synaptophysin fusion protein Ag11781|
|Calculated molecular weight||313 aa, 34 kDa|
|Observed molecular weight||38-40 kDa|
|GenBank accession number||BC064550|
|Gene ID (NCBI)||6855|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
|Product Specific Protocols|
|WB protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|IHC protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|IF protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|IP protocol for Synaptophysin antibody 17785-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (05-31-2021)
Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)