TAOK1 Polyclonal antibody

TAOK1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 26250-1-AP

Synonyms

hKFC B, KIAA1361, Kinase from chicken homolog B, MAP3K16, MARKK, PSK2, STE20 like kinase PSK2, TAO kinase 1, TAO1, TAOK1

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Validation Data Gallery

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  WB analysis of HeLa using 26250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26250-1-AP (TAOK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26250-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26250-1-AP (TAOK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26250-1-AP (TAOK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26250-1-AP (TAOK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26250-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26250-1-AP (TAOK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 5 publications below

Product Information

26250-1-AP targets TAOK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAOK1 fusion protein Ag22461
• Full Name: TAO kinase 1
• Calculated Molecular Weight: 1001 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC133039
• Gene Symbol: TAOK1
• Gene ID (NCBI): 57551
• RRID: AB_2880446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thousand and one kinase 1 (TAOK1), also known as prostate-derived sterile 20 (Ste20)-like kinase 2, TAO1 (thousand and one amino-acid protein 1) or MARKK, is a member of the MAP3K protein kinase and belongs to the germinal-center kinase-like class of sterile 20 (Ste20)-like kinases (PMID: 29400705). TAOK1 is involved in mammalian brain development and functions as a negative regulator in IL-17-mediated signaling and inflammation (PMID: 33565190). TAOK1 has a calculated molecular mass of 116 kDa and encodes a serine/threonine protein kinase at its N terminus.

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.; Authors - David T Burns
human	WB,IF	Acta Neuropathol Commun; A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.; Authors - Caterina Giacomini
mouse	IF	Sci Signal; Neurodevelopmental disorder-associated mutations in TAOK1 reveal its function as a plasma membrane remodeling kinase; Authors - Neal Beeman
human	WB,IF	Mol Cancer Ther; Targeting TAO kinases using a new inhibitor compound delays mitosis and induces mitotic cell death in centrosome amplified breast cancer cells.; Authors - Chuay-Yeng Koo
monkey	WB	J Cell Sci; Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning.; Authors - Ritu Garg
human	WB,IF	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj