Validation Data Gallery
|Positive WB detected in||Caco-2 cells, K-562 cells|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human testis tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
22337-1-AP targets TCF4 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, Drosophila|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TCF4 fusion protein Ag17813|
|Full Name||transcription factor 4|
|Calculated molecular weight||671 aa, 72 kDa|
|Observed molecular weight||72 kDa|
|GenBank accession number||BC125084|
|Gene ID (NCBI)||6925|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Transcription factor 4 (TCF4), also known as SEF2, ITF2, E2-2, and ME2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Human TCF4 mRNA expression is particularly high in the brain. Usage of numerous 5' exons of the human TCF4 gene potentially yields in TCF4 protein isoforms with 18 different N-termini. In addition, the diversity of isoforms is increased by alternative splicing of several internal exons. Some isoforms contain a bipartite nuclear localization signal and are exclusively nuclear, whereas distribution of other isoforms relies on heterodimerization partners. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). The scopel of molecular weight of several isoforms is about 60-90 kDa (21789225).
|Product Specific Protocols|
|WB protocol for TCF4 antibody 22337-1-AP||Download protocol|
|IHC protocol for TCF4 antibody 22337-1-AP||Download protocol|
|IF protocol for TCF4 antibody 22337-1-AP||Download protocol|
|IP protocol for TCF4 antibody 22337-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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