TCF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

TCF4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

56

Cat no : 22337-1-AP

Synonyms

ITF-2, ITF2, ITF 2, E2 2, Class B basic helix-loop-helix protein 19

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Validation Data Gallery

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  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 22337-1-AP

  IP result of anti-TCF4 (IP:22337-1-AP, 4ug; Detection:22337-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse brain using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22337-1-AP (TCF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22337-1-AP (TCF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:200.

• 

  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:200.

• 

  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:50.

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TCF4 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TCF4 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22337-1-AP (TCF4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22337-1-AP (TCF4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells, K-562 cells
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 47 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• ChIP: See 3 publications below

Product Information

22337-1-AP targets TCF4 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCF4 fusion protein Ag17813
• Full Name: transcription factor 4
• Calculated Molecular Weight: 671 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC125084
• Gene Symbol: TCF4
• Gene ID (NCBI): 6925
• RRID: AB_2879076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor 4 (TCF4), also known as SEF2, ITF2, E2-2, and ME2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Human TCF4 mRNA expression is particularly high in the brain. Usage of numerous 5' exons of the human TCF4 gene potentially yields in TCF4 protein isoforms with 18 different N-termini. In addition, the diversity of isoforms is increased by alternative splicing of several internal exons. Some isoforms contain a bipartite nuclear localization signal and are exclusively nuclear, whereas distribution of other isoforms relies on heterodimerization partners. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). The scopel of molecular weight of several isoforms is about 60-90 kDa (21789225).

Publications

Species	Application	Title
	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Nat Commun; Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.; Authors - Norman L Lehman
	WB,IF,ChIP	Acta Pharmacol Sin; Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction; Authors - Jing Li
human	IHC	JCI Insight; Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling; Authors - Yanyun Jiang
human,Drosophila	WB,IF	Cell Rep; Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.; Authors - Mitchell D'Rozario ; KD Validated

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