• Featured Product
  • KD/KO Validated

TDP-43 (C-terminal) Monoclonal antibody

TDP-43 (C-terminal) Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5F6

Cat no : 67345-1-Ig

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43, TDP-43 (C-terminal)



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67345-1-Ig targets TDP-43 (C-terminal) in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.

Protocols

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
FC protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Neurochem Int

PARP-1 activation leads to cytosolic accumulation of TDP-43 in neurons.

Authors - Joshua M Marcus
humanWB,IF

Biomedicines

TDP-43 Pathology and Prionic Behavior in Human Cellular Models of Alzheimer's Disease Patients.

Authors - Eva P Cuevas