TDP-43 (C-terminal) Monoclonal antibody

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• KD/KO Validated

TDP-43 (C-terminal) Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G5F6

Cat no : 67345-1-Ig

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43, TDP-43 (C-terminal)

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Validation Data Gallery

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  WB analysis of HeLa using 67345-1-Ig

  WB result of TDP-43 (C-terminal) antibody (67345-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 (C-terminal) transfected HeLa cells.

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  WB analysis using 67345-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67345-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67345-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67345-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 67345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67345-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.20ug Anti-Human TDP-43 (C-terminal) (67345-1-Ig, Clone:1G5F6) (red) or 0.20 ug Mouse IgG2a Isotype Control(66360-2-Ig, CloneK11A1B2A2) (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Y79 cells, SH-SY5Y cells, Neuro-2a cells, PC-12 cells
• Positive IHC detected in: mouse brain tissue, human gliomas tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat brain tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:20000
• Immunofluorescence (IF): IF : 1:250-1:1000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 4 publications below

Product Information

67345-1-Ig targets TDP-43 (C-terminal) in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TARDBP
• Gene ID (NCBI): 23435
• RRID: AB_2882603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.

Publications

Species	Application	Title
human	WB,IF	Biomedicines; TDP-43 Pathology and Prionic Behavior in Human Cellular Models of Alzheimer's Disease Patients.; Authors - Eva P Cuevas
human	IF	Neurochem Int; PARP-1 activation leads to cytosolic accumulation of TDP-43 in neurons.; Authors - Joshua M Marcus
human,mouse	WB,IF	ASN Neuro; Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia; Authors - Michael Ohene-Nyako
human	WB,IF	Front Mol Neurosci; TTBK1 and CK1 inhibitors restore TDP-43 pathology and avoid disease propagation in lymphoblast from Alzheimer's disease patients; Authors - Loreto Martinez-Gonzalez