TDP-43 (C-terminal) Monoclonal antibody

TDP-43 (C-terminal) Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G5F6

Cat no : 67345-1-Ig

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

67345-1-Ig targets TDP-43 (C-terminal) in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.

Protocols

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
FC protocol for TDP-43 (C-terminal) antibody 67345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Neurochem Int

PARP-1 activation leads to cytosolic accumulation of TDP-43 in neurons.

Authors - Joshua M Marcus