Tested Applications
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67345 targets TDP-43 (C-terminal) in IF-P applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TAR DNA binding protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43-45 kDa |
| GenBank Accession Number | NM_007375 |
| Gene Symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_2889840 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13148 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TDP-43 (C-terminal) antibody CL488-67345 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TDP-43 (C-terminal) Monoclonal antibody (clone 1G5F6), catalog number CL488-67345, has a total of 1 citation. It appears in bioRxiv, a preprint server. The associated research field is neurodegenerative disease, specifically amyotrophic lateral sclerosis (ALS), examining how dysregulated lactate metabolism synergizes with ALS genetic risk factors to accelerate motor decline. The antibody was applied for immunofluorescence (IF) using mouse samples.
| Species | Application | Title |
|---|---|---|
bioRxiv Dysregulated lactate metabolism synergizes with ALS genetic risk factors to accelerate motor decline. |
