CoraLite®594-conjugated TDP-43 (C-terminal) Monoclonal antibody

TDP-43 (C-terminal) Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G5F6

Cat no : CL594-67345

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

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Validation Data Gallery

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  IF Staining of rat brain using CL594-67345

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594-conjugated TDP-43 (C-terminal) antibody (CL594-67345, Clone: 1G5F6 ) at dilution of 1:300.

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Tested Applications

• Positive IF detected in: rat brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67345 targets TDP-43 (C-terminal) in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TARDBP
• Gene ID (NCBI): 23435
• RRID: AB_2889841
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.