TGFB1I1 Recombinant antibody

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  WB analysis of HeLa using 85859-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (TGFB1I1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 85859-5-RR

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (TGFB1I1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 85859-5-RR

  rat colon tissue were subjected to SDS PAGE followed by western blot with 85859-5-RR (TGFB1I1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TGFB1I1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85859-5-RR

Clone No.250139E8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

HIC5, Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein

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Tested Applications

• Positive WB detected in: HeLa cells, rat colon tissue, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85859-5-RR targets TGFB1I1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TGFB1I1 fusion protein Ag0868
• Full Name: transforming growth factor beta 1 induced transcript 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC001830
• Gene Symbol: HIC5
• Gene ID (NCBI): 7041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIC5 (Hydrogen Peroxide-Inducible Clone 5), also known as TGFB1I1, ARA55, or TSC-5. It was first identified in 1994 as a gene induced by transforming growth factor-β (TGF-β) or hydrogen peroxide (PMID: 36222304). HIC5 is a member of the Paxillin protein family and functions as a molecular adapter, delivering signals when the cellular adhesion environment changes. Given its role in cancer and fibrotic diseases, HIC5 is a potential therapeutic target. For example, targeting HIC5 or the GR tau2 region could modulate the set of genes regulated by GR, providing opportunities for intervention (PMID: 24591583).