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TRAF2 Monoclonal antibody, PBS Only

TRAF2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4D1

Cat no : 67315-1-PBS

Synonyms

TRAF2, TRAP, TRAP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAF2 Monoclonal antibody (67315-1-Ig)

    WB analysis using 67315-1-Ig (same clone as 67315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TRAF2 Monoclonal antibody (67315-1-Ig)

    WB analysis of HepG2 using 67315-1-Ig (same clone as 67315-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAF2 Monoclonal antibody (67315-1-Ig)

    IF Staining of HeLa using 67315-1-Ig (same clone as 67315-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67315-1-PBS targets TRAF2 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TRAF2 fusion protein Ag25387
Full Name TNF receptor-associated factor 2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC032410
Gene Symbol TRAF2
Gene ID (NCBI) 7186
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.