TRAF2 Monoclonal antibody

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  WB analysis using 67315-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HepG2 using 67315-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67315-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

TRAF2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67315-1-Ig

Clone No.2A4D1

Publications(4)

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

TRAF2, TRAP, TRAP3

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, THP-1 cells, K-562 cells, Jurkat cells, L02 cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

67315-1-Ig targets TRAF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25387

  Product name: Recombinant human TRAF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 438-501 aa of BC032410

  Sequence: NNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL

• Full Name: TNF receptor-associated factor 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC032410
• Gene Symbol: TRAF2
• Gene ID (NCBI): 7186
• RRID: AB_2882575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12933
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cell Rep; Liquidambaric acid inhibits Wnt/β-catenin signaling and colon cancer via targeting TNF receptor-associated factor 2.; Authors - Rong Yan
human	WB,IP	FEBS J; TRAF2 associates with cullin neddylation complex assembly; Authors - Tiantian Wang ; KO Validated
human	WB	J Ethnopharmacol; Extract of Nanhaia speciosa J. Compton & Schrire alleviates LPS-induced acute lung injury via the NF-κB/Nrf2/AQPs pathway; Authors - Shiyao Zhou
mouse	WB	Int J Biol Macromol; Bilobalide ameliorates ovariectomy-induced osteoporosis by influencing SIRT3/NF-κB axis in osteoclast and promoting M2 polarization in macrophages; Authors - YiFang Qin