TRIM59 antibody (28575-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 28575-1-AP (same clone as 28575-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.
WB analysis using 28575-1-AP (same clone as 28575-1-PBS)
rat spleen tissue were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 28575-1-AP (same clone as 28575-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 28575-1-AP (same clone as 28575-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 28575-1-AP (same clone as 28575-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 28575-1-AP (same clone as 28575-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 28575-1-AP (same clone as 28575-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM59 antibody (28575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

28575-1-PBS targets TRIM59 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIM59 fusion protein Ag29560 Predict reactive species
Full Name	tripartite motif-containing 59
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	NM_173084
Gene Symbol	TRIM59
Gene ID (NCBI)	286827
RRID	AB_2918177
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IWR1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TRIM59 belongs to the tripartite motif (TRIM) family. It functions as E3 ubiquitin ligases or an adaptor protein, and plays important roles in various types of human cancers.