TRIM59 Polyclonal antibody

TRIM59 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 28575-1-AP

Synonyms

MRF1, RING finger protein 104, RNF104, TRIM57, TRIM59, tripartite motif containing 59, TSBF1, Tumor suppressor TSBF 1



Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inhuman pancreas cancer tissue, human liver tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28575-1-AP targets TRIM59 in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM59 fusion protein Ag29560
Full Name tripartite motif-containing 59
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberNM_173084
Gene Symbol TRIM59
Gene ID (NCBI) 286827
RRIDAB_2918177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM59 belongs to the tripartite motif (TRIM) family. It functions as E3 ubiquitin ligases or an adaptor protein, and plays important roles in various types of human cancers.

Protocols

Product Specific Protocols
WB protocol for TRIM59 antibody 28575-1-APDownload protocol
IHC protocol for TRIM59 antibody 28575-1-APDownload protocol
IF protocol for TRIM59 antibody 28575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP,ChIP

J Transl Med

A role of TRIM59 in pulmonary hypertension: modulating the protein ubiquitylation modification

Authors - Yingli Liu