CoraLite® Plus 647 Anti-Human TWEAKR/CD266 Rabbit Recombinant Antibody

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  FC experiment of HT-1080 using CL647-98125

  1x10^6 HT-1080 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human TWEAKR/CD266 Rabbit RecAb (CL647-98125, Clone:240991C5)(red) or 5 ul CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

TWEAKR/CD266 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98125

Clone No.240991C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD266, TNFRSF12A, TweakR, 240991C5, APO3L

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Tested Applications

• Positive FC detected in: HT-1080 cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98125 targets TWEAKR/CD266 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TWEAKR/CD266 fusion protein Ag13823
• Full Name: tumor necrosis factor receptor superfamily, member 12A
• Calculated Molecular Weight: 129 aa, 14 kDa
• GenBank Accession Number: BC002718
• Gene Symbol: TWEAKR
• Gene ID (NCBI): 51330
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NP84
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)