UBE2D1/2/3/4 Polyclonal antibody

UBE2D1/2/3/4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28328-1-AP

Synonyms

E2(17)KB2, PUBC1, UBC4, UBC4/5, UBC5B, UBCH4, UBCH5B, UBE2D1, UBE2D2, UBE2D3, UBE2D4, Ubiquitin carrier protein D2, Ubiquitin protein ligase D2

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Validation Data Gallery

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  WB analysis using 28328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 28328-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28328-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, NIH/3T3 cells, mouse testis tissue, mouse kidney tissue, rat kidney tissue, PC-12 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28328-1-AP targets UBE2D1/2/3/4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2D1/2/3/4 fusion protein Ag28736
• Full Name: ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: BC033349
• Gene Symbol: UBE2D2
• Gene ID (NCBI): 7322
• RRID: AB_2881114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

Publications

Species	Application	Title
mouse	WB	Nat Commun; CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I; Authors - Cheng-Jie Zhou